C6992
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 | Whole cell lysates were separated on SDS-PAGE and blotted with Anti-CENP-A (C-terminal) (Cat. No. C6992). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1. HEK293 cells (untransfected) 2, 3. HEK293 cells transfected with human CENP-A 3. Inhibition with CENP-A (human 124-140) immunizing peptide (20 μg/mL) |
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C6992
Sigma
Anti-CENP-A (C-terminal) antibody produced in rabbit
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-CenH3, Anti-Centromere Protein A |
Description
| Immunogen | synthetic peptide corresponding to amino acids 124-140 located at the C-terminus of human CENP-A, conjugated to KLH. This sequence is highly conserved (88% identity) in bovine and dog CENP-A, and shows 60% identity with histone H3. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 17 kDa |
| species reactivity | human |
| concentration | ~2 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using HEK-293 cells expressing human CENP-A |
| Gene Information | human ... CENPA(1058) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
